![]() ![]() This needs to be followed up in further studies,” said Poisel. Interestingly, differential DNA methylation was related to several transcription factors and proteins with DNA binding domains, which implies direct effects of these DNA methylation changes on gene expression. “We were surprised that in our network analysis changes in DNA methylation were especially prominent among genes that regulate the activity of neurons and the connectivity between them. ![]() They found 17 genomic regions that were more methylated in donors with CUD than in donors without CUD, and three regions that were less methylated in donors with CUD than in donors without CUD. They corrected for differences in the donor’s age, the time since death, the brain pH, and further diseases such as depressive disorder and alcohol use disorder. Poisel and colleagues also looked at differences in the degree of methylation at 654,448 sites in the human genome, and looked for associations with the presence or absence of CUD in the life of each donor. “As biological age estimation is a very recent concept in addiction research and is influenced by many factors, further studies are required to investigate this phenomenon, with larger sample sizes than were possible here.” Associations between CUD and methylated genes This could be caused by cocaine-related disease processes in the brain, such as inflammation or cell death,” said lead author Dr Stephanie Witt, a researcher at the same institute. “We detected a trend towards stronger biological aging of the brain in individuals with cocaine use disorder compared to individuals without cocaine use disorder. Scientists can thus estimate the biological age from methylome data with established mathematical algorithms. The biological age of cells, tissues, and organs can be greater or less than their chronological age, depending on diet, lifestyle, and exposure to disease or harmful environmental factors. Here, they used patterns of DNA methylation as a measure of the biological age of cells in Brodmann Area 9. The researchers found evidence that cells in Brodmann Area 9 appear biologically ‘older’ in people with CUD, suggesting that these cells age faster than in people without substance use disorders. Brain cells may age faster in people addicted to cocaine ![]() This is important, because most earlier studies in this field were done on the brains of rats. “As DNA methylation is an important regulatory mechanism for gene expression, the identified DNA methylation alterations might contribute to functional changes in the human brain and thereby to the associated behavioral aspects of addiction,” said first author Eric Poisel, a PhD student at the Central Institute of Mental Health in Mannheim, Germany.īecause the study of the brain methylome is invasive, the study was done on the cryo-preserved brains of 42 deceased male donors, of whom half had had CUD while the other half had not. Typically, a greater degree of DNA methylation leads to the ‘dialing down’ of nearby genes. Now, scientists from Germany and Canada have shown in Frontiers in Psychiatry that in humans, cocaine use disorder (CUD) leads to changes in the ‘methylome’ of a subregion within the prefrontal cortex, Brodmann Area 9, thought to be important for self-awareness and inhibitory control. But what are the biochemical changes in the prefrontal cortex that cause this impairment? In contrast, such ‘inhibitory control’ is impaired in the addicted brain, making it hard to resist. Healthy brains aren’t at the mercy of such dopamine rushes, however: there, the prefrontal cortex weighs options and can decide to forgo pleasurable activities when it’s not the time or place. Drugs like cocaine copy this effect, except up to ten times more strongly. When we enjoy sex, food, music, or hobbies, regions of our brain within the reward pathway are flooded with pleasure-inducing dopamine. ![]() Scientists tend to view substance addiction as primarily a disease of the brain. This post-mortem study is one of the first to directly look at the methylome of brain cells in human subjects with cocaine use disorder, rather than in rodents. The authors also find differences in methylation in 20 genes, mainly involved in regulation of the activity of neurons and their connectivity. This suggests that cocaine abuse makes these cells age faster according to the ‘epigenetic clock’. By Mischa Dijkstra, Frontiers science writerĪ new study finds evidence from the DNA methylome that the biological age – different from the chronological age – of cells in Brodmann Area 9 of the prefrontal cortex might be greater in people with cocaine use disorder. ![]()
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